19 декабря, 2022 
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Overall performance
1.3 Gbp) were processed to remove SMRT bell adapters, short and low-quality reads (< 80% accuracy) using SMRT Analysis version 2.3 ( A total of 155,828 filtered reads (average length, 8.9 Kb) were used for de novo assembly using Celera Assembler , with self-correction of the PacBio reads. A total of 1091 Mb paired-end Illumina reads screened from a 500-bp genomic library were mapped using SOAP to the bacterial plasmid database for tracing the presence of any plasmid and filling the gaps. The de novo assembly resulted in five contigs, representing the K. michiganensis BD177 complete genome of 6,812,698 bp with GC content % in a single chromosome and four plasmids. The annotated genome contains 6714 genes, 25rRNA genes, and 86 tRNA genes (Table 1).
16S rRNA phylogenetic data and you may phenotypic from remote filter systems BD177
Maximum parsimony depending and you may restriction opportunities phylogenetic trees utilising the sequence you to definitely encodes for the 16S rRNA gene revealed that brand new closest species so you can BD177 is actually K. michiganensis and K. oxytica (Extra document step one: Fig. S1, Additional file 2: Dining table S1). Evolutionary divergence point out of 16S rRNA gene series anywhere between BD177 and K. michiganensis E718 is minimum value (BD177 vs K. michiganensis E718: 0.0079) certainly the sequences. Biochemical evaluation had been did towards the BD177 because of the API20E to verify the status on K. oxytoca and you can K. michiganensis. Biochemical attributes out-of K. oxytoca ATCC13182(T)(=NCTC13727) and you may K. michiganensis W14(T)(= DSM2544) was basically taken from BacDive Webservices pared that have K. Читать далее »